g Senescent cells were co-stained by SPiDER SA-β Gal, and the LIVE/DEAD Near-IR dead cell stain kit then analyzed by flow cytometry under indicated conditions. h Quantification of dead senescent cells at indicated conditions, as in (g). Data represent mean ± SD of n = 3 biologically independent experiments. P values were calculated using a two-tailed Student’s t test. Source data are provided as a Source Data file.
https://www.nature.com/articles/s41467-024-49745-5/figures/6
不懂就问,这篇文章里不是使用One-way ANOVA更好?!
实验设计 | 统计方法 | 目的 | 例子 |
---|---|---|---|
两个因素,每个有多个水平 | Two-way ANOVA + 事后检验 (Sidak's) | 分析主效应和交互效应 | 基因型 x 处理条件 |
一个因素,三个及以上水平 | One-way ANOVA + 事后检验 | 比较多个独立组的差异 | 多种处理方式 |
两个独立组 | Unpaired t-test | 比较两个组的差异 | WT vs KO 的存活率 |
数据不满足参数检验前提 | Mann-Whitney U (两组) 或 Kruskal-Wallis (多组) | 非参数替代方法,比较中位数 |
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原创声明:本文系作者授权腾讯云开发者社区发表,未经许可,不得转载。
如有侵权,请联系 cloudcommunity@tencent.com 删除。