#每组分别计算不同mismatch的标准偏差及坐标
library(tidyverse)
E_A = x %>% filter(Type == "E",primer=="A") %>%
group_by...for_sd=unlist(c(100,E_A[2,2],E_A[2,2]+E_A[3,2])))
E_B = x %>% filter(Type == "E",primer=="B") %>%
group_by...unlist(c(100,E_C[2,2],E_C[2,2]+E_C[3,2])) )
EK_A = x %>% filter(Type == "EK",primer=="A") %>%
group_by...=unlist(c(100,S_A[2,2],S_A[2,2]+S_A[3,2])) )
S_B = x %>% filter(Type == "S",primer=="B") %>%
group_by...unlist(c(100,S_B[2,2],S_B[2,2]+S_B[3,2])) )
S_C = x %>% filter(Type == "S",primer=="C") %>%
group_by