生信入门 第七天

This is day 7 homework, the last one for BIC by 生信星球.

The content for today is the introduction of DNA sequencing.

1. three generations of DNA sequencing technologies

1.1 1st generation: Sanger method is the most successful one.

1.2 2nd generation: NGS, is currently one of the most popular techniques.

Roche-454 GS FLX

Illumina solexa genome analyzer

ABI SOLiD sequencer

1.3 3rd generation: none-emplication, long reading, single molecule sequencing, however high erra rate at 10-15%

PacBio SMRT

Oxford Naopore Technologies

This is a good review from Genomics 2016 Jan;107(1):1-8.

The sequence of sequencers: The history of sequencing DNA by

James M Heather, Benjamin Chainfiliations expand

DOI: 10.1016/j.ygeno.2015.11.003

The end